Recombinant Enterobacteria phage lambda Host-nuclease inhibitor protein gam (gam), Escherichia phage lambda (Bacteriophage lambda), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 23.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Enterobacteria phage lambda Host-nuclease inhibitor protein gam (gam)

Recombinant Enterobacteria phage lambda Host-nuclease inhibitor protein gam (gam) — Both GamL and GamS bind to host RecBCD nuclease and inhibit its activities, protecting the viral DNA against RecBCD mediated degradation. Purity >85%.

SKU: BCRECP-002815 Category:
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Product Specifications

Product SkuBCRECP-002815
Product DescriptionRecombinant Enterobacteria phage lambda Host-nuclease inhibitor protein gam (gam) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-138aa. [Accession | P03702].
Uniprot No.P03702
Gene Namesgam
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-138aa
SpeciesEscherichia phage lambda (Bacteriophage lambda)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight23.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Both GamL and GamS bind to host RecBCD nuclease and inhibit its activities, protecting the viral DNA against RecBCD mediated degradation. Isoform GamS mimics DNA, preventing RecBCD from binding DNA. Expression of this protein in E.coli renders cells more sensitive to fluoroquinolone antibiotics (which inhibit topoisomerases and induce dsDNA breaks).

Product Specifications

Recombinant Enterobacteria phage lambda Host-nuclease inhibitor protein gam (gam) is a recombinant protein from Escherichia phage lambda (Bacteriophage lambda), expressed in E.coli, covering amino acids 1-138aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 23.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Both GamL and GamS bind to host RecBCD nuclease and inhibit its activities, protecting the viral DNA against RecBCD mediated degradation (PubMed:126236, PubMed:1653221, PubMed:17544443, PubMed:2943636, PubMed:4275917). Isoform GamS mimics DNA, preventing RecBCD from binding DNA (PubMed:17544443, PubMed:28009252). Expression of this protein in E.coli renders cells more sensitive to fluoroquinolone antibiotics (which inhibit topoisomerases and induce dsDNA breaks) (PubMed:28009252). Frameshift mutations that delete most of this protein (starting from position 78 or 97 of GamL) allow the bacteriophage to overcome the retron Ec48 bacteriophage defense system. Overexpression of the wild-type gam protein in E.coli with an intact Ec48 retron leads to a dramatic decrease in cell growth, the frameshift mutants do not have this effect (PubMed:33157039)

Subunit

Homodimer (PubMed:126236, PubMed:17544443, PubMed:28009252). Interacts with host RecBCD without displacing any of the subunits; this interaction prevents RecBCD from binding to DNA (PubMed:1653221, PubMed:17544443, PubMed:28009252). The GamS isoform makes extensive contacts with RecB, and inserts deeply into the RecBCD complex (PubMed:28009252)

Gene: gam  |  Organism: Escherichia phage lambda
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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