Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated, Gallus gallus (Chicken), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 83.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated

Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated — Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Purity >90%.

SKU: BCRECP-002818 Categories: , ,
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Product Specifications

Product SkuBCRECP-002818
Product DescriptionRecombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 2-333aa. [Accession | P00356].
Uniprot No.P00356
Gene NamesGAPDH
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-333aa
SpeciesGallus gallus (Chicken)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight83.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Product Specifications

Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated is a recombinant protein from Gallus gallus (Chicken), expressed in E.coli, covering amino acids 2-333aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 83.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)

Subcellular Location

Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus

Subunit

Homotetramer

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Gene: GAPDH  |  Organism: Gallus gallus  |  Synonyms: Peptidyl-cysteine S-nitrosylase GAPDH
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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