Recombinant Trichoderma reesei Hydrophobin-2 (hfb2), Hypocrea jecorina (Trichoderma reesei), Yeast, N-terminal 6xHis-sumostar-tagged, 23.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Trichoderma reesei Hydrophobin-2 (hfb2)

Recombinant Trichoderma reesei Hydrophobin-2 (hfb2) — Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence i… Purity >90%.

SKU: BCRECP-002985 Category:
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Product Specifications

Product SkuBCRECP-002985
Product DescriptionRecombinant Trichoderma reesei Hydrophobin-2 (hfb2) Protein is expressed from Yeast with N-terminal 6xHis-sumostar-tagged. It contains 16-86aa. [Accession | P79073].
Uniprot No.P79073
Gene Nameshfb2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region16-86aa
SpeciesHypocrea jecorina (Trichoderma reesei)
Tag InfoN-terminal 6xHis-sumostar-tagged
Molecular weight23.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence identity. Class I can self-assemble into an outermost layer of rodlet bundles on aerial cell surfaces, conferring cellular hydrophobicity that supports fungal growth, development and dispersal; whereas Class II form highly ordered films at water-air interfaces through intermolecular interactions but contribute nothing to the rodlet structure (Probable). Hbf2 is a class II hydrophobin that is involved in sporulation.

Biological Context

Subcellular Location: Secreted; Spore wall; Secreted, cell wall

Product Specifications

Recombinant Trichoderma reesei Hydrophobin-2 (hfb2) is a recombinant protein from Hypocrea jecorina (Trichoderma reesei), expressed in Yeast, covering amino acids 16-86aa, with N-terminal 6xHis-sumostar-tagged tag, molecular weight 23.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence identity. Class I can self-assemble into an outermost layer of rodlet bundles on aerial cell surfaces, conferring cellular hydrophobicity that supports fungal growth, development and dispersal; whereas Class II form highly ordered films at water-air interfaces through intermolecular interactions but contribute nothing to the rodlet structure (Probable). Hbf2 is a class II hydrophobin that is involved in sporulation (PubMed:16243453, PubMed:9346297)

Subcellular Location

Secreted; Spore wall; Secreted, cell wall

Subunit

Homodimer (PubMed:17636262). Homodimers further self-assemble to form highly ordered films at water-air interfaces through intermolecular interactions (PubMed:17636262)

Gene: hfb2  |  Organism: Trichoderma reesei  |  Synonyms: Hydrophobin II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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