Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 3 (hmw3), partial, Mycoplasma pneumoniae (strain ATCC 29342 / M129), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 3 (hmw3), partial

Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 3 (hmw3), partial — Component of the cytoskeleton-like structure which stabilizes the shape of the wall-less mycoplasma. Purity >85%.

SKU: BCRECP-003004 Category:
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Product Specifications

Product SkuBCRECP-003004
Product DescriptionRecombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 3 (hmw3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 160-339aa. [Accession | Q50360].
Uniprot No.Q50360
Gene Nameshmw3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region160-339aa
SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Component of the cytoskeleton-like structure which stabilizes the shape of the wall-less mycoplasma. This cytoskeleton-like network of accessory proteins allows the proper anchoring of cytadhesin proteins in the mycoplasmal membrane at the attachment organelle. The attachment organelle is involved in gliding cell motility and adhesion to host cells.

Biological Context

Subcellular Location: Cell projection, attachment organelle; Cytoplasm, cytoskeleton

Product Specifications

Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 3 (hmw3), partial is a recombinant protein from Mycoplasma pneumoniae (strain ATCC 29342 / M129), expressed in E.coli, covering amino acids 160-339aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the cytoskeleton-like structure which stabilizes the shape of the wall-less mycoplasma (PubMed:26633540). This cytoskeleton-like network of accessory proteins allows the proper anchoring of cytadhesin proteins in the mycoplasmal membrane at the attachment organelle. The attachment organelle is involved in gliding cell motility and adhesion to host cells

Subcellular Location

Cell projection, attachment organelle; Cytoplasm, cytoskeleton

Gene: hmw3  |  Organism: Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)  |  Synonyms: Accessory adhesin protein 3; Cytadherence accessory protein 3

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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