Recombinant Synechococcus elongatus Circadian clock protein kinase kaiC (kaiC), Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 65.6kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Synechococcus elongatus Circadian clock protein kinase kaiC (kaiC)

Recombinant Synechococcus elongatus Circadian clock protein kinase kaiC (kaiC) — recombinant protein from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), expressed in E.coli. Purity >95%.

SKU: BCRECP-003207 Categories: , ,
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Product Specifications

Product SkuBCRECP-003207
Product DescriptionRecombinant Synechococcus elongatus Circadian clock protein kinase kaiC (kaiC) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-519aa. [Accession | Q79PF4].
Uniprot No.Q79PF4
Gene NameskaiC
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-519aa
SpeciesSynechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight65.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

KaiC is important for metabolic partitioning during the dark to light shift, modulating the balance between the Calvin cycle and oxidative pentose phosphate pathway under natural growth conditions.

Product Specifications

Recombinant Synechococcus elongatus Circadian clock protein kinase kaiC (kaiC) is a recombinant protein from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2), expressed in E.coli, covering amino acids 1-519aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 65.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

KaiC is important for metabolic partitioning during the dark to light shift, modulating the balance between the Calvin cycle and oxidative pentose phosphate pathway under natural growth conditions

Subunit

Homohexamer resembling 2 stacked donuts with a central pore nearly blocked on one side; hexamerization is dependent on ATP-binding. Binds 12 ATP; 6 between each subunit in both layers (PubMed:15304218, PubMed:15347809, PubMed:22304631, PubMed:24474762, PubMed:35427168). KaiB only binds to phospho-Ser-431 KaiC (not doubly phosphorylated KaiC) (PubMed:17717528, PubMed:29892030). Complex formation between KaiB and KaiC is regulated by the phosphorylation state of KaiC and by an ATP hydrolysis-driven conformation change in the CI ring of KaiC; complex formation is slow. Slow complex formation is crucial for the timing of the circadian period (PubMed:29892030). KaiB switches to a thioredoxin-like form called KaiB(fs) when bound to KaiC (PubMed:26113641). The KaiABC complex composition changes during the circadian cycle to control KaiC phosphorylation. Complexes KaiC(6), KaiA(2-4):KaiC(6), KaiB(6):KaiC(6) and KaiC(6):KaiB(6):KaiA(12) are among the most important forms, many form cooperatively (PubMed:28302852, PubMed:34618577). Interacts directly with KaiB and SasA (PubMed:10786837). The CI domain binds to KaiB and SasA; as they have a similar fold they compete for the same site on CI (PubMed:29892030, PubMed:34618577). CikA interacts with this protein in the clock complex (PubMed:17088557). Binds to the C-terminus of KaiA via a coiled-coil structure (PubMed:26200123). Forms KaiC(6):KaiB(1) and KaiC(6):KaiB(6) complexes (PubMed:23796516, PubMed:24474762)

Gene: kaiC  |  Organism: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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