Recombinant Pig NKG2-D type II integral membrane protein (KLRK1), partial, Sus scrofa (Pig), E.coli, C-terminal 6xHis-tagged, 22.9kDa, purity >95% as determined by SDS-PAGE.

Recombinant Pig NKG2-D type II integral membrane protein (KLRK1), partial

Recombinant Pig NKG2-D type II integral membrane protein (KLRK1), partial — Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and… Purity >95%.

SKU: BCRECP-003221 Category:
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Product Specifications

Product SkuBCRECP-003221
Product DescriptionRecombinant Pig NKG2-D type II integral membrane protein (KLRK1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 78-214aa. [Accession | Q9GLF5].
Uniprot No.Q9GLF5
Gene NamesKLRK1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region78-214aa
SpeciesSus scrofa (Pig)
Tag InfoC-terminal 6xHis-tagged
Molecular weight22.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation.

Biological Context

Subcellular Location: Cell membrane (Single-pass type II membrane protein)
Tissue Specificity: Detected in peripheral blood leukocytes, macrophages, monocytes and natural killer cells

Product Specifications

Recombinant Pig NKG2-D type II integral membrane protein (KLRK1), partial is a recombinant protein from Sus scrofa (Pig), expressed in E.coli, covering amino acids 78-214aa, with C-terminal 6xHis-tagged tag, molecular weight 22.9kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins (By similarity)

Subcellular Location

Cell membrane (Single-pass type II membrane protein)

Tissue Specificity

Detected in peripheral blood leukocytes, macrophages, monocytes and natural killer cells

Subunit

Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Can form disulfide-bonded heterodimer with CD94 (By similarity). Interacts with CEACAM1; recruits PTPN6 that dephosphorylates VAV1 (By similarity)

Gene: KLRK1  |  Organism: Sus scrofa  |  Synonyms: Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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