Recombinant Mouse Lactase-like protein (Lctl), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 64.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Lactase-like protein (Lctl), partial

Recombinant Mouse Lactase-like protein (Lctl), partial — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >90%.

SKU: BCRECP-003246 Category:
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Product Specifications

Product SkuBCRECP-003246
Product DescriptionRecombinant Mouse Lactase-like protein (Lctl) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-540aa. [Accession | Q8K1F9].
Uniprot No.Q8K1F9
Gene NamesLctl
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-540aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight64.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass membrane protein)
Tissue Specificity: Strongly expressed in the lens of the eye, where it localizes to the equatorial epithelium and outer layers of newly extending fiber cells (at protein level). May also be expressed in kidney and skin. However, another study suggests that expression is specific to eye and is minimal in other tissues

Product Specifications

Recombinant Mouse Lactase-like protein (Lctl), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 21-540aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 64.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens

Subcellular Location

Endoplasmic reticulum membrane (Single-pass membrane protein)

Tissue Specificity

Strongly expressed in the lens of the eye, where it localizes to the equatorial epithelium and outer layers of newly extending fiber cells (at protein level) (PubMed:29425878). May also be expressed in kidney and skin (PubMed:12084582). However, another study suggests that expression is specific to eye and is minimal in other tissues (PubMed:29425878)

Subunit

May form dimers

Gene: Lctl  |  Organism: Mus musculus  |  Synonyms: Klotho/lactase-phlorizin hydrolase-related protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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