Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA), partial, Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans), Yeast, N-terminal 6xHis-tagged, 38.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA), partial

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA), partial — Virulence factor that plays an important role in immune evasion. Purity >85%.

SKU: BCRECP-003312 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003312
Product DescriptionRecombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 721-1055aa. [Accession | P16462].
Uniprot No.P16462
Gene NamesltxA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region721-1055aa
SpeciesAggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans)
Tag InfoN-terminal 6xHis-tagged
Molecular weight38.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Virulence factor that plays an important role in immune evasion. Lyses human lymphocytes and monocytes. Binds to the LFA-1 integrin on the surface of the host cell and to cholesterol-containing membranes, which probably results in large LtxA-LFA-1 clusters in lipid rafts.

Biological Context

Subcellular Location: Cell outer membrane (Peripheral membrane protein); Secreted

Product Specifications

Recombinant Aggregatibacter actinomycetemcomitans Leukotoxin (ltxA), partial is a recombinant protein from Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans), expressed in Yeast, covering amino acids 721-1055aa, with N-terminal 6xHis-tagged tag, molecular weight 38.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Virulence factor that plays an important role in immune evasion. Lyses human lymphocytes and monocytes. Binds to the LFA-1 integrin on the surface of the host cell and to cholesterol-containing membranes, which probably results in large LtxA-LFA-1 clusters in lipid rafts. Also shows beta-hemolytic activity on certain types of growth media

Subcellular Location

Cell outer membrane (Peripheral membrane protein); Secreted

Subunit

Interacts specifically with the superoxide dismutase [Cu-Zn]. This interaction may protect LtxA from reactive oxygen species and reactive nitrogen species produced by host inflammatory cells during disease (PubMed:17635874). Interacts with the human leukocyte adhesion glycoprotein LFA-1 (ITGAL-ITGB2) (PubMed:17635865)

Gene: ltxA  |  Organism: Aggregatibacter actinomycetemcomitans
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top