Mouse anti-Human/Mouse/Rat TUBB1 Monoclonal Antibody

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

Subcellular Location

Cytoplasm, cytoskeleton

Disease Association

Macrothrombocytopenia, isolated, 1, autosomal dominant (MACTHC1) : A congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins

Subunit

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with RANBP10

• Gross C. et al. Mapping and characterization of the human TUBB1 gene. (JUL-)
• Deloukas P. et al. The DNA sequence and comparative analysis of human chromosome 20. — Nature (2001)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Gevaert K. et al. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. — Nat. Biotechnol. (2003)
• Fourest-Lieuvin A. et al. Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1. — Mol. Biol. Cell (2006)
• Schulze H. et al. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. — J. Biol. Chem. (2008)
• Rogowski K. et al. Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. — Cell (2009)
• Leandro-Garcia L.J. et al. Tumoral and tissue-specific expression of the major human beta-tubulin isotypes. — Cytoskeleton (2010)