Recombinant Human Ubiquitin-associated domain-containing protein 1 (UBAC1) (Active), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 72.3 kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Ubiquitin-associated domain-containing protein 1 (UBAC1) (Active)

Recombinant Human Ubiquitin-associated domain-containing protein 1 (UBAC1) (Active) — Non-catalytic component of the KPC complex, a E3 ubiquitin-protein ligase complex that mediates polyubiquitination of target proteins, such as CDKN1B and NFKB1. Purity >85%.

SKU: BCACP-000001 Category:
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Product SKUBCACP-000001
Product DescriptionRecombinant Human Ubiquitin-associated domain-containing protein 1 (UBAC1) protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-405aa. [Accession | Q9BSL1].
Uniprot No.Q9BSL1
Gene NamesUBAC1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-405aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight72.3 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human UBAC1, the EC50 of human UBAC1 protein is 86.67-97.25 μg/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesUBAC1; GBDR1; KPC2; UBADC1; Ubiquitin-associated domain-containing protein 1; UBA domain-containing protein 1; E3 ubiquitin-protein ligase subunit KPC2; Glialblastoma cell differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2

Function

Non-catalytic component of the KPC complex, a E3 ubiquitin-protein ligase complex that mediates polyubiquitination of target proteins, such as CDKN1B and NFKB1. The KPC complex catalyzes polyubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle. The KPC complex also acts as a key regulator of the NF-kappa-B signaling by promoting maturation of the NFKB1 component of NF-kappa-B by catalyzing ubiquitination of the NFKB1 p105 precursor.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Ubiquitous
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human Ubiquitin-associated domain-containing protein 1 (UBAC1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-405aa, with N-terminal GST-tagged tag, molecular weight 72.3 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Non-catalytic component of the KPC complex, a E3 ubiquitin-protein ligase complex that mediates polyubiquitination of target proteins, such as CDKN1B and NFKB1 (PubMed:15531880, PubMed:15746103, PubMed:16227581, PubMed:25860612). The KPC complex catalyzes polyubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle (PubMed:15531880, PubMed:15746103). The KPC complex also acts as a key regulator of the NF-kappa-B signaling by promoting maturation of the NFKB1 component of NF-kappa-B by catalyzing ubiquitination of the NFKB1 p105 precursor (PubMed:25860612). Within the KPC complex, UBAC1 acts as an adapter that promotes the transfer of target proteins that have been polyubiquitinated by RNF123/KPC1 to the 26S proteasome (PubMed:16227581)

Subcellular Location

Cytoplasm

Tissue Specificity

Ubiquitous

Subunit

Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2 (PubMed:15531880, PubMed:15746103, PubMed:16227581). Interacts (via ubiquitin-like domain) with RNF123 (PubMed:15746103, PubMed:16227581). Interacts (via ubiquitin-like and UBA domains) with the proteasome via its N-terminal domain (PubMed:15746103, PubMed:16227581)

Pathway

Protein modification; protein ubiquitination

Gene: UBAC1  |  Organism: Homo sapiens  |  Synonyms: Glialblastoma cell differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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