Recombinant Human Mucin-16 (MUC16), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 30.5 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Mucin-16 (MUC16), partial (Active)

Recombinant Human Mucin-16 (MUC16), partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000102 Category:
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Product SKUBCACP-000102
Product DescriptionRecombinant Human Mucin-16 (MUC16) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 12660-12923aa. [Accession | Q8WXI7].
Uniprot No.Q8WXI7
Gene NamesMUC16
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region12660-12923aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight30.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNF-α at 5 μg/ml can bind human TNFR1, the EC50 is 7.799-10.90 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized LTA at 5 μg/ml can bind human TNFR1, the EC50 is 4.409-6.797 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(MUC-16)(Ovarian cancer-related tumor marker CA125)(CA-125)(CA125)

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Secreted, extracellular space
Tissue Specificity: Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas

Product Specifications

Recombinant Human Mucin-16 (MUC16), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 12660-12923aa, with C-terminal 6xHis-tagged tag, molecular weight 30.5 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Secreted, extracellular space

Tissue Specificity

Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas

Subunit

Binds to MSLN. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN

Gene: MUC16  |  Organism: Homo sapiens  |  Synonyms: Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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