Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active), Mus musculus (Mouse), Mammalian cell, C-terminal mFc-tagged, 108.1 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active)

Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active) — Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Purity >95%.

SKU: BCACP-000118 Category:
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Product SKUBCACP-000118
Product DescriptionRecombinant Mouse Semaphorin-4D (Sema4d) protein is expressed from Mammalian cell with C-terminal mFc-tagged. It contains 24-733aa. [Accession | O09126].
Uniprot No.O09126
Gene NamesSema4d
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region24-733aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal mFc-tagged
Molecular weight108.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Mouse Gfral at 5 μg/mL can bind Mouse Gdf15 , the EC50 is 7.926-10.52 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. Expressed in neurons and glia in the developing hippocampus

Product Specifications

Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 24-733aa, with C-terminal mFc-tagged tag, molecular weight 108.1 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed:23699507, PubMed:29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed:23699507, PubMed:29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed:17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues (PubMed:8969198). Expressed in neurons and glia in the developing hippocampus (PubMed:29981480)

Subunit

Homodimer (By similarity). Interacts with PLXNB1 (By similarity). Interacts with PLXNB2 (PubMed:17554007)

Gene: Sema4d  |  Organism: Mus musculus  |  Synonyms: M-Sema G; Semaphorin-C-like 2; Semaphorin-J
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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