Recombinant Dog Angiopoietin-2 (ANGPT2) (Active), Canis lupus familiaris (Dog) (Canis familiaris), Mammalian cell, C-terminal 6xHis-tagged, 57.2 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Dog Angiopoietin-2 (ANGPT2) (Active)

Recombinant Dog Angiopoietin-2 (ANGPT2) (Active) — Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Purity >95%.

SKU: BCACP-000187 Category:
Online Inquiry →
Product SKUBCACP-000187
Product DescriptionRecombinant Canis familiaris Angiopoietin-2 (ANGPT2) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 19-495aa. [Accession | A0A8J8].
Uniprot No.A0A8J8
Gene NamesANGPT2
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region19-495aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoC-terminal 6xHis-tagged
Molecular weight57.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/mL can bind anti-ENPP3 recombinant antibody , the EC50 is 2.151-2.492 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(ANG-2)

Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression.

Product Specifications

Recombinant Dog Angiopoietin-2 (ANGPT2) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 19-495aa, with C-terminal 6xHis-tagged tag, molecular weight 57.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (By similarity). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (By similarity). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression (By similarity). In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Involved in the regulation of lymphangiogenesis (By similarity)

Gene: ANGPT2  |  Organism: Canis lupus familiaris

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top