Recombinant Human V-set domain-containing T-cell activation inhibitor 1 (VTCN1) , partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 27.2 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.

Recombinant Human V-set domain-containing T-cell activation inhibitor 1 (VTCN1) , partial (Active)

Recombinant Human V-set domain-containing T-cell activation inhibitor 1 (VTCN1) , partial (Active) — Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. Purity >95%.

SKU: BCACP-000395 Category:
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Product SKUBCACP-000395
Product DescriptionRecombinant Human V-set domain-containing T-cell activation inhibitor 1 (VTCN1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 29-258aa. [Accession | Q7Z7D3].
Uniprot No.Q7Z7D3
Gene NamesVTCN1
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region29-258aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight27.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITGAV&ITGB6 at 2 μg/mL can bind Macaca mulatta TGFB1 (M)). The EC50 is 8.899-14.82 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ITGAV&ITGB6 at 2 μg/mL can bind Anti-ITGB6 recombinant antibody. The EC50 is 4.251-5.103 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesV-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7-H4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x

Function

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Product Specifications

Recombinant Human V-set domain-containing T-cell activation inhibitor 1 (VTCN1) , partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 29-258aa, with C-terminal 10xHis-tagged tag, molecular weight 27.2 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation

Gene: VTCN1  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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