Recombinant Human Legumain (LGMN) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 49.0 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Legumain (LGMN) (Active)

Recombinant Human Legumain (LGMN) (Active) — Has a strict specificity for hydrolysis of asparaginyl bonds. Purity >95%.

SKU: BCACP-000591 Category:
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Product SKUBCACP-000591
Product DescriptionRecombinant Human Legumain (LGMN) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 18-433aa. [Accession | Q99538].
Uniprot No.Q99538
Gene NamesLGMN
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region18-433aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight49.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human LGALS3 at 2 μg/mL can bind anti-LGALS3 recombinant antibody.The EC50 is 2.361-2.785 ng/mL.;LGALS3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human LGALS3 with an affinity constant of 7.54 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesLegumain; EC 3.4.22.34; Asparaginyl endopeptidase; AEP; Protease, cysteine 1

Function

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Biological Context

Subcellular Location: Lysosome
Tissue Specificity: Ubiquitous. Particularly abundant in kidney, heart and placenta

Product Specifications

Recombinant Human Legumain (LGMN) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 18-433aa, with C-terminal 10xHis-tagged tag, molecular weight 49.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed:9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity)

Subcellular Location

Lysosome

Tissue Specificity

Ubiquitous. Particularly abundant in kidney, heart and placenta

Subunit

Homodimer before autocatalytic removal of the propeptide (PubMed:23776206). Monomer after autocatalytic processing (PubMed:23776206). May interact with integrins (PubMed:23776206)

Gene: LGMN  |  Organism: Homo sapiens  |  Synonyms: Asparaginyl endopeptidase; Protease, cysteine 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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