Recombinant Mouse Delta-like protein 3 (Dll3), partial (Active), Mus musculus (Mouse), Mammalian cell, N-terminal HSA-tagged and C-terminal 10xHis-tagged, 115.7 kDa, purity >95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.

Recombinant Mouse Delta-like protein 3 (Dll3), partial (Active)

Recombinant Mouse Delta-like protein 3 (Dll3), partial (Active) — Inhibits primary neurogenesis. Purity >95%.

SKU: BCACP-000643 Category:
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Product SKUBCACP-000643
Product DescriptionRecombinant Mouse Delta-like protein 3 (Dll3) protein is expressed from Mammalian cell with N-terminal HSA-tagged and C-terminal 10xHis-tagged. It contains 33-490aa. [Accession | O88516].
Uniprot No.O88516
Gene NamesDll3
PurityGreater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region33-490aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal HSA-tagged and C-terminal 10xHis-tagged
Molecular weight115.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCN2 at 1 μg/mL can bind Anti-CCN2 recombinant antibody . The EC50 is 0.7088-0.8299 ng/mL.;CCN2 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human CCN2 with an affinity constant of 6.43 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesDelta-like protein 3; Drosophila Delta homolog 3; Delta3; M-Delta-3

Function

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

Biological Context

Subcellular Location: Membrane (Single-pass type I membrane protein)
Tissue Specificity: Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis
Disease Association: [A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities]

Product Specifications

Recombinant Mouse Delta-like protein 3 (Dll3), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 33-490aa, with N-terminal HSA-tagged and C-terminal 10xHis-tagged tag, molecular weight 115.7 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm

Subcellular Location

Membrane (Single-pass type I membrane protein)

Disease Association

[A truncating mutation in Dll3 is the cause of the pudgy (pu) phenotype. Pudgy mice exhibit patterning defects at the earliest stages of somitogenesis. Adult pudgy mice present severe vertebral and rib deformities]

Tissue Specificity

Predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis

Subunit

Can bind and activate Notch-1 or another Notch receptor

Gene: Dll3  |  Organism: Mus musculus  |  Synonyms: Drosophila Delta homolog 3

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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