Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16), Mus musculus (Mouse), E.Coli, Tag-Free, 12.2 kDa, purity >>98% as determined by SDS-PAGE.
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Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16)

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) — Isoform 2 is involved in cell cycle progression in T-cells. Purity >98%.

SKU: BCACP-000722 Categories: ,
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Product SKUBCACP-000722
Product DescriptionRecombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) protein is expressed from E.Coli with Tag-Free. It contains 1205-1322aa. [Accession | O54824].
Uniprot No.O54824
Gene NamesIl16
Purity>98% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region1205-1322aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight12.2 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x106 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl16; Pro-interleukin-16 [Cleaved into: Interleukin-16; IL-16; Lymphocyte chemoattractant factor; LCF)]

Function

Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung

Product Specifications

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 1205-1322aa, with Tag-Free tag, molecular weight 12.2 kDa, purity >98% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung

Subunit

Homotetramer (Probable). Isoform 2 interacts with GRIN2A. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C. Isoform 2 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GABPB1. Isoform 2 interacts (via PDZ 3 domain) with HDAC3 (By similarity)

Gene: Il16  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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