Recombinant Human Beta-defensin 1 protein (DEFB1) (Active), Homo sapiens (Human), E.Coli, Tag-Free, 5.1 kDa, purity >>98% as determined by SDS-PAGE.

Recombinant Human Beta-defensin 1 protein (DEFB1) (Active)

Recombinant Human Beta-defensin 1 protein (DEFB1) (Active) — Has bactericidal activity. Purity >98%.

SKU: BCACP-000887 Category:
Online Inquiry →
Product SKUBCACP-000887
Product DescriptionRecombinant Human Beta-defensin 1 protein (DEFB1) protein is expressed from E.Coli with Tag-Free. It contains 22-68aa. [Accession | P60022].
Uniprot No.P60022
Gene NamesDEFB1
Purity>98% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region22-68aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight5.1 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesBD 1; BD-1; BD1; beta 1; Beta defensin 1; Beta-defensin 1; DEFB 1; DEFB1; DEFB1_HUMAN; DEFB101; Defensin; Defensin beta 1; Defensin beta 1 preproprotein; HBD 1; hBD-1; HBD1; MGC51822

Function

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner.

Biological Context

Subcellular Location: Secreted; Membrane
Tissue Specificity: Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level)

Product Specifications

Recombinant Human Beta-defensin 1 protein (DEFB1) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 22-68aa, with Tag-Free tag, molecular weight 5.1 kDa, purity >98% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:25122636)

Subcellular Location

Secreted; Membrane

Tissue Specificity

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level)

Subunit

Monomer (PubMed:23938203). Homodimer (PubMed:23938203)

Gene: DEFB1  |  Organism: Homo sapiens  |  Synonyms: Defensin, beta 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top