Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC) Protein, Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV), in vitro E.coli expression system, N-terminal 10xHis-tagged, 57.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC) Protein

Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC) Protein — Forms the virion spikes together with glycoprotein G1. Purity >85%.

SKU: BCRECP-00039TM Categories: ,
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Product Specifications

Uniprot No.P08669
Gene NamesGPC
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region2-491aa
SpeciesLassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV)
Tag InfoN-terminal 10xHis-tagged
Molecular weight57.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Product Specifications

Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC) Protein is a recombinant protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV), expressed in in vitro E.coli expression system, covering amino acids 2-491aa, with N-terminal 10xHis-tagged tag, molecular weight 57.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms the virion spikes together with glycoprotein G1 (PubMed:26849049, PubMed:35173332). The glycoprotein spike trimers are connected to the underlying matrix (PubMed:26849049). Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification (PubMed:31004664)

Subcellular Location

Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Subunit

Homotrimer (PubMed:28572385, PubMed:31004664, PubMed:31398326, PubMed:31456769, PubMed:35173332). Interacts with the stable signal peptide (PubMed:14555961, PubMed:35173332). In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions (PubMed:35173332). Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide (Probable) (PubMed:28572385, PubMed:35173332). Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state (PubMed:28572385, PubMed:31004664, PubMed:31456769). The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding (By similarity)

Gene: GPC  |  Organism: Lassa virus (strain Mouse/Sierra Leone/Josiah/1976)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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