Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein, Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC), in vitro E.coli expression system, N-terminal 10xHis-tagged, 56.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein — Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. Purity >85%.

SKU: BCRECP-00132TM Category:
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Product Specifications

Uniprot No.Q8FIB8
Gene NamesphoQ
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-486aa
SpeciesEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Tag InfoN-terminal 10xHis-tagged
Molecular weight56.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. Also involved in adaptation to hyperosmotic environments, in a Mg(2+)-independent manner. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG).

Biological Context

Subcellular Location: Cell inner membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Escherichia coli O6:H1 Sensor protein PhoQ (phoQ) Protein is a recombinant protein from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC), expressed in in vitro E.coli expression system, covering amino acids 1-486aa, with N-terminal 10xHis-tagged tag, molecular weight 56.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Member of the two-component regulatory system PhoP/PhoQ involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes (By similarity). Also involved in adaptation to hyperosmotic environments, in a Mg(2+)-independent manner (By similarity). In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG) (By similarity). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, which results in the repression of PG and may lead to expression of some PRG (By similarity)

Subcellular Location

Cell inner membrane (Multi-pass membrane protein)

Subunit

Homodimer; probably dimerizes via the cytoplasmic domain. Probably interacts with MgrB in the periplasm, altering its activity and that of downstream effector PhoP. Interacts with two-component system connector protein SafA (via C-terminus)

Gene: phoQ  |  Organism: Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)  |  Synonyms: Sensor histidine protein kinase/phosphatase PhoQ

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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