Recombinant Human G-protein coupled receptor 182 (GPR182) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 46.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human G-protein coupled receptor 182 (GPR182) Protein

Recombinant Human G-protein coupled receptor 182 (GPR182) Protein — Atypical chemokine receptor that regulates chemokine levels and localization through chemokine binding, independently of activating classical ligand-induced signaling pathways such as G protein act… Purity >85%.

SKU: BCRECP-00172TM Category:
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Product Specifications

Uniprot No.O15218
Gene NamesGPR182
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-404aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight46.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Atypical chemokine receptor that regulates chemokine levels and localization through chemokine binding, independently of activating classical ligand-induced signaling pathways such as G protein activation and Ca(2+) mobilization. Instead, it mediates chemokine sequestration, transport, or internalization to control their availability. Acts as a scavenger for a broad range of chemokines from CXC, CC and XC chemokine ligand families including CXCL9, CXCL10, CXCL12, CXCL13, CXCL11, CXCL14, CCL1, CCL11, CCL19, CCL25, CCL28 and XCL1.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Endosome membrane (Multi-pass membrane protein)
Tissue Specificity: Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver

Product Specifications

Recombinant Human G-protein coupled receptor 182 (GPR182) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-404aa, with N-terminal 10xHis-tagged tag, molecular weight 46.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Atypical chemokine receptor that regulates chemokine levels and localization through chemokine binding, independently of activating classical ligand-induced signaling pathways such as G protein activation and Ca(2+) mobilization (PubMed:33875597, PubMed:35013216, PubMed:37554323, PubMed:38026988). Instead, it mediates chemokine sequestration, transport, or internalization to control their availability (PubMed:33875597, PubMed:35013216, PubMed:37554323, PubMed:38026988). Acts as a scavenger for a broad range of chemokines from CXC, CC and XC chemokine ligand families including CXCL9, CXCL10, CXCL12, CXCL13, CXCL11, CXCL14, CCL1, CCL11, CCL19, CCL25, CCL28 and XCL1 (PubMed:33875597, PubMed:35013216, PubMed:38026988). Is the only atypical receptor for chemokines CXCL13 and CCL28 (PubMed:37554323). Has a strong constitutive association with beta-arrestins, which is essential for intracellular receptor trafficking and chemokine scavenging, and occurs independently of ligand binding (PubMed:33875597, PubMed:37554323). Cooperates with ACKR3 and ACKR4 in regulating serum levels of CXCL12 and CCL19, respectively (PubMed:37554323). Acts as an important modulator of cellular immunity (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Endosome membrane (Multi-pass membrane protein)

Tissue Specificity

Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver

Subunit

Strong constitutive association with beta-arrestins; leading to internalization of ACKR5 and its ligands

Gene: ACKR5  |  Organism: Homo sapiens  |  Synonyms: G protein-coupled receptor 182
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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