Recombinant Mouse G-protein coupled receptor 171 (Gpr171) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 42.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse G-protein coupled receptor 171 (Gpr171) Protein

Recombinant Mouse G-protein coupled receptor 171 (Gpr171) Protein — G protein-coupled receptor for Big LEN, a 16-amino acid neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Purity >85%.

SKU: BCRECP-00190TM Category:
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Product Specifications

Uniprot No.Q8BG55
Gene NamesGpr171
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-319aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight42.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor for Big LEN, a 16-amino acid neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)-alpha-mediated pathway in response to Big LEN. Big LEN-GPR171 system plays an important role in regulating feeding and metabolism.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Highly expressed in hypothalamus, including the arcuate nucleus, paraventricular nucleus and dorsomedial hypothalamus. Expressed in periaqueductal gray (at protein level), found primarily in GABAergic neurons and to a lesser extent in glutamatergic neurons. Expressed in T cells and natural killer cells

Product Specifications

Recombinant Mouse G-protein coupled receptor 171 (Gpr171) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 1-319aa, with N-terminal 10xHis-tagged tag, molecular weight 42.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for Big LEN, a 16-amino acid neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N) (PubMed:24043826, PubMed:28425495). Acts through a G(i)-alpha-mediated pathway in response to Big LEN (PubMed:24043826). Big LEN-GPR171 system plays an important role in regulating feeding and metabolism (PubMed:24043826). Also plays a role in modulating fear and anxiety-like behaviors in the basolateral amygdala (PubMed:28425495). Big LEN-GPR171 modulates the mu-type opioid receptor signaling and antinociception (PubMed:31308196). Acts as a negative regulator T cell function (PubMed:34615877)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Highly expressed in hypothalamus, including the arcuate nucleus, paraventricular nucleus and dorsomedial hypothalamus (PubMed:24043826). Expressed in periaqueductal gray (at protein level), found primarily in GABAergic neurons and to a lesser extent in glutamatergic neurons (PubMed:31308196). Expressed in T cells and natural killer cells (PubMed:34615877)

Gene: Gpr171  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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