Recombinant Rat Synaptotagmin-1 (Syt1) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 10xHis-tagged, 53.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Synaptotagmin-1 (Syt1) Protein

Recombinant Rat Synaptotagmin-1 (Syt1) Protein — (Microbial infection) Receptor for C. Purity >85%.

SKU: BCRECP-00254TM Category:
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Product Specifications

Uniprot No.P21707
Gene NamesSyt1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-421aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight53.5kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

(Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); unlike the case with BoNT/B, interaction is not improved in the presence of gangliosides. BoNT/G toxin binds to the vesicular domain of Syt1 (residues 32-53).

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane (Single-pass membrane protein); Cytoplasm
Tissue Specificity: Expressed in the brain (at protein level). Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues

Product Specifications

Recombinant Rat Synaptotagmin-1 (Syt1) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-421aa, with N-terminal 10xHis-tagged tag, molecular weight 53.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

(Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); unlike the case with BoNT/B, interaction is not improved in the presence of gangliosides (PubMed:15123599, PubMed:20219474). BoNT/G toxin binds to the vesicular domain of Syt1 (residues 32-53) (PubMed:15123599, PubMed:20219474)

Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane (Single-pass membrane protein); Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane (Single-pass membrane protein); Cytoplasm

Tissue Specificity

Expressed in the brain (at protein level) (PubMed:17190793). Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues

Subunit

(Microbial infection) Interacts with C.botulinum neurotoxin type G (BoNT/G, botG)

Gene: Syt1  |  Organism: Rattus norvegicus  |  Synonyms: Synaptotagmin I; p65
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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