Recombinant Human G protein-activated inward rectifier potassium channel 1 (KCNJ3)(F137S) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 62.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human G protein-activated inward rectifier potassium channel 1 (KCNJ3)(F137S) Protein

Recombinant Human G protein-activated inward rectifier potassium channel 1 (KCNJ3)(F137S) Protein — Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Purity >85%.

SKU: BCRECP-00366TM Category:
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Product Specifications

Uniprot No.P48549
Gene NamesKCNJ3
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-501aa(F137S)
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight62.6kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Biological Context

Subcellular Location: Membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Human G protein-activated inward rectifier potassium channel 1 (KCNJ3)(F137S) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-501aa(F137S), with N-terminal 10xHis-tagged tag, molecular weight 62.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This potassium channel is controlled by G proteins (PubMed:8804710, PubMed:8868049). This receptor plays a crucial role in regulating the heartbeat (By similarity)

Subcellular Location

Membrane (Multi-pass membrane protein)

Subunit

Associates with KCNJ5/GIRK4 or KCNJ6/GIRK2 to form a G protein activated heteromultimer pore-forming unit. The resulting inward current is much larger (PubMed:10659995, PubMed:8868049). Associates with KCNJ9/GIRK3 to form a G protein activated heteromultimer pore-forming unit (By similarity)

Gene: KCNJ3  |  Organism: Homo sapiens  |  Synonyms: Inward rectifier K(+) channel Kir3.1; Potassium channel, inwardly rectifying subfamily J member 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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