Recombinant Bovine Rhodopsin (RHO) Protein, Bos taurus (Bovine), in vitro E.coli expression system, C-terminal 10xHis-tagged, 42.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bovine Rhodopsin (RHO) Protein

Recombinant Bovine Rhodopsin (RHO) Protein — G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions. Purity >85%.

SKU: BCRECP-00498TM Category:
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Product Specifications

Uniprot No.P02699
Gene NamesRHO
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-348aa
SpeciesBos taurus (Bovine)
Tag InfoC-terminal 10xHis-tagged
Molecular weight42.0kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions. Required for postnatal photoreceptor cell viability. Activation occurs when the covalently bound 11-cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers G protein-mediated phototransduction (Probable).

Biological Context

Subcellular Location: Photoreceptor outer segment membrane (Multi-pass membrane protein); Membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in rod-shaped photoreceptor cells in the retina that mediate vision in dim light (at protein level)

Product Specifications

Recombinant Bovine Rhodopsin (RHO) Protein is a recombinant protein from Bos taurus (Bovine), expressed in in vitro E.coli expression system, covering amino acids 1-348aa, with C-terminal 10xHis-tagged tag, molecular weight 42.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions (PubMed:31300275). Required for postnatal photoreceptor cell viability (By similarity). Activation occurs when the covalently bound 11-cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers G protein-mediated phototransduction (Probable) (PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:31300275). Signal termination occurs via receptor phosphorylation, which promotes binding of arrestin (SAG) and displacement of the G protein alpha subunit (Probable) (PubMed:1396673)

Subcellular Location

Photoreceptor outer segment membrane (Multi-pass membrane protein); Membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in rod-shaped photoreceptor cells in the retina that mediate vision in dim light (at protein level)

Subunit

Homodimer (PubMed:18563085, PubMed:23303210). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (PubMed:17020884). Interacts with GRK1 (By similarity). Interacts (phosphorylated form) with SAG (PubMed:15111114, PubMed:15351781, PubMed:23579341, PubMed:25205354, PubMed:26200343). Interacts with GNAT1 (PubMed:18818650, PubMed:21389983, PubMed:23303210, PubMed:23579341, PubMed:26526852, PubMed:28655769, PubMed:31300275). Interacts with GNAT3 (PubMed:22198838, PubMed:27458239). SAG and G proteins compete for a common binding site (By similarity). Interacts with PRCD; the interaction promotes PRCD stability (PubMed:27509380). Forms a complex with ASAP1 and ARF4 (PubMed:25673879). Forms a complex with ASAP1, RAB11A, Rabin8/RAB3IP, ARF4 and RAB11FIP3; the complex regulates Golgi-to-cilia rhodopsin/RHO transport in photoreceptors (PubMed:25673879)

Gene: RHO  |  Organism: Bos taurus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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