Recombinant Human Vasopressin V2 receptor (AVPR2) Protein, in vitro E.coli expression system, N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged, 44.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Vasopressin V2 receptor (AVPR2) Protein

Recombinant Human Vasopressin V2 receptor (AVPR2) Protein — G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption. Purity >85%.

SKU: BCRECP-00582TM Category:
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Product Specifications

Uniprot No.P30518
Gene NamesAVPR2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-371aa
Tag InfoN-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged
Molecular weight44.8kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). AVPR2 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Kidney
Disease Association: Nephrogenic syndrome of inappropriate antidiuresis (NSIAD) : Characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. [The disease is caused by variants affecting the gene represented in this entry] | Diabetes insipidus, nephrogenic, 1, X-linked (NDI1) : A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Vasopressin V2 receptor (AVPR2) Protein is a recombinant protein, expressed in in vitro E.coli expression system, covering amino acids 1-371aa, with N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged tag, molecular weight 44.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption (PubMed:1534149, PubMed:19440390, PubMed:33664408, PubMed:33742150). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (PubMed:33664408, PubMed:33742150). AVPR2 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed:33664408, PubMed:33742150)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Disease Association

Nephrogenic syndrome of inappropriate antidiuresis (NSIAD) : Characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. [The disease is caused by variants affecting the gene represented in this entry] | Diabetes insipidus, nephrogenic, 1, X-linked (NDI1) : A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Kidney

Subunit

Interacts with ARRDC4 (PubMed:23236378). Identified in a complex containing at least ARRDC4, V2R and HGS (PubMed:23236378). Interacts with TMEM147 (PubMed:21056967). Interacts (when phosphorylated) with ARRB2; the interaction is associated with internalization of the receptor and short-term desensitization to the ligand (PubMed:37209686)

Gene: AVPR2  |  Organism: Homo sapiens  |  Synonyms: AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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