Recombinant Human E3 ubiquitin-protein ligase MARCH2 (MARCH2) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 43.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human E3 ubiquitin-protein ligase MARCH2 (MARCH2) Protein

Recombinant Human E3 ubiquitin-protein ligase MARCH2 (MARCH2) Protein — (Microbial infection) Positively regulates the degradation of Vesicular stomatitis virus (VSV) G protein via the lysosomal degradation pathway. Purity >90%.

SKU: BCRECP-00609TM Categories: ,
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Product Specifications

Uniprot No.Q9P0N8
Gene Names2-Mar
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-246aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight43.0kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

(Microbial infection) Positively regulates the degradation of Vesicular stomatitis virus (VSV) G protein via the lysosomal degradation pathway. Represses HIV-1 viral production and may inhibit the translocation of HIV-1 env to the cell surface, resulting in decreased viral cell-cell transmission.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein); Endosome membrane (Multi-pass membrane protein); Golgi apparatus membrane (Multi-pass membrane protein); Cytoplasm; Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Broadly expressed
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Human E3 ubiquitin-protein ligase MARCH2 (MARCH2) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-246aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 43.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Positively regulates the degradation of Vesicular stomatitis virus (VSV) G protein via the lysosomal degradation pathway (PubMed:29573664). Represses HIV-1 viral production and may inhibit the translocation of HIV-1 env to the cell surface, resulting in decreased viral cell-cell transmission (PubMed:29573664)

Subcellular Location

Endoplasmic reticulum membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein); Endosome membrane (Multi-pass membrane protein); Golgi apparatus membrane (Multi-pass membrane protein); Cytoplasm; Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Broadly expressed

Subunit

Interacts with STX6; the interaction promotes MARCHF2-mediated ubiquitination and degradation of CFTR (PubMed:23818989). Interacts with MARCHF3 (PubMed:16428329). Interacts with GOPC/CAL; the interaction leads to CFTR ubiquitination and degradation (PubMed:23818989). Interacts with CFTR; the interaction leads to CFTR ubiquitination and degradation (PubMed:23818989). Interacts (via PDZ domain) with DLG1 (via PDZ domains); the interaction leads to DLG1 ubiquitination and degradation (PubMed:17980554). Interacts with ERGIC3 (PubMed:31142615). Interacts with ADRB2 (PubMed:23166351). Interacts with IKBKG/NEMO; during the late stages of macrophage viral and bacterial infection; the interaction leads to ubiquitination and degradation of IKBKG/NEMO (PubMed:32935379)

Pathway

Protein modification; protein ubiquitination

Gene: MARCHF2  |  Organism: Homo sapiens  |  Synonyms: Membrane-associated RING finger protein 2; Membrane-associated RING-CH protein II; RING finger protein 172; RING-type E3 ubiquitin transferase MARCHF2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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