Recombinant Human B-cell CLL/lymphoma 9 protein (BCL9), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged and C-terminal 6xHis-tagged, 26.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human B-cell CLL/lymphoma 9 protein (BCL9), partial

Recombinant Human B-cell CLL/lymphoma 9 protein (BCL9), partial — Involved in signal transduction through the Wnt pathway. Purity >85%.

SKU: BCRECP-000156 Category:
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Product Specifications

Product SkuBCRECP-000156
Product DescriptionRecombinant Human B-cell CLL/lymphoma 9 protein (BCL9) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged. It contains 174-396aa. [Accession | O00512].
Uniprot No.O00512
Gene NamesBCL9
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region174-396aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Molecular weight26.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood
Disease Association: [A chromosomal aberration involving BCL9 is found in a patient with precursor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies]

Product Specifications

Recombinant Human B-cell CLL/lymphoma 9 protein (BCL9), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 174-396aa, with N-terminal 6xHis-tagged and C-terminal 6xHis-tagged tag, molecular weight 26.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity)

Subcellular Location

Nucleus

Disease Association

[A chromosomal aberration involving BCL9 is found in a patient with precursor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies]

Tissue Specificity

Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood

Subunit

Binds to beta-catenin (CTNNB1), PYGO1 and PYGO2; the interaction with PYGO1 increases PYGO1 affinity to histone H3 methylated at 'Lys 4'

Gene: BCL9  |  Organism: Homo sapiens  |  Synonyms: Protein legless homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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