Recombinant Human Calpain small subunit 1 (CAPNS1), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 44.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Calpain small subunit 1 (CAPNS1)

Recombinant Human Calpain small subunit 1 (CAPNS1) — Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Purity >90%.

SKU: BCRECP-000233 Category:
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Product Specifications

Product SkuBCRECP-000233
Product DescriptionRecombinant Human Calpain small subunit 1 (CAPNS1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-268aa. [Accession | P04632].
Uniprot No.P04632
Gene NamesCAPNS1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-268aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight44.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development.

Biological Context

Subcellular Location: Cytoplasm; Cell membrane
Disease Association: Pulmonary hypertension, primary, 6 (PPH6) : A form of primary pulmonary hypertension, a disease defined by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. Primary pulmonary hypertension exhibits incomplete penetrance, sex bias and variable age of onset, both within and between families. PPH6 is an autosomal recessive form. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Calpain small subunit 1 (CAPNS1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-268aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 44.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity)

Subcellular Location

Cytoplasm; Cell membrane

Disease Association

Pulmonary hypertension, primary, 6 (PPH6) : A form of primary pulmonary hypertension, a disease defined by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. Primary pulmonary hypertension exhibits incomplete penetrance, sex bias and variable age of onset, both within and between families. PPH6 is an autosomal recessive form. [The disease may be caused by variants affecting the gene represented in this entry]

Subunit

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity)

Gene: CAPNS1  |  Organism: Homo sapiens  |  Synonyms: Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit; Calcium-dependent protease small subunit 1; Calpain regulatory subunit

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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