Recombinant Human Dihydropyrimidinase-related protein 1 (CRMP1), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 78.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Dihydropyrimidinase-related protein 1 (CRMP1)

Recombinant Human Dihydropyrimidinase-related protein 1 (CRMP1) — Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Purity >90%.

SKU: BCRECP-000430 Category:
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Product Specifications

Product SkuBCRECP-000430
Product DescriptionRecombinant Human Dihydropyrimidinase-related protein 1 (CRMP1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-572aa. [Accession | Q14194].
Uniprot No.Q14194
Gene NamesCRMP1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-572aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight78.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cell projection, growth cone; Cytoplasm, cytoskeleton; Perikaryon
Tissue Specificity: Brain

Product Specifications

Recombinant Human Dihydropyrimidinase-related protein 1 (CRMP1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-572aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 78.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed:25358863). Plays a role in axon guidance (PubMed:25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed:25358863). Involved in invasive growth and cell migration (PubMed:11562390). May participate in cytokinesis (PubMed:19799413)

Subcellular Location

Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cell projection, growth cone; Cytoplasm, cytoskeleton; Perikaryon

Tissue Specificity

Brain

Subunit

Homotetramer, and heterotetramer with DPYSL2, DPYSL3, DPYSL4 or DPYSL5 (By similarity). Interacts with PLXNA1 (By similarity). Interacts with FLNA (via calponin-homology (CH) domain 1 and filamin repeat 24); the interaction alters FLNA ternary structure and thus promotes FLNA dissociation from F-actin (PubMed:25358863)

Gene: CRMP1  |  Organism: Homo sapiens  |  Synonyms: Collapsin response mediator protein 1; Inactive dihydropyrimidinase; Unc-33-like phosphoprotein 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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