Recombinant Human D (2) dopamine receptor (DRD2), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 25.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human D (2) dopamine receptor (DRD2), partial

Recombinant Human D (2) dopamine receptor (DRD2), partial — Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Purity >85%.

SKU: BCRECP-000535 Category:
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Product Specifications

Product SkuBCRECP-000535
Product DescriptionRecombinant Human D (2) dopamine receptor (DRD2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 214-373aa. [Accession | P14416].
Uniprot No.P14416
Gene NamesDRD2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region214-373aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight25.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasG-protein coupled receptor, Receptor, Transducer

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Golgi apparatus membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in the anterior pituitary gland

Product Specifications

Recombinant Human D (2) dopamine receptor (DRD2), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 214-373aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 25.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase (PubMed:21645528, PubMed:39103320). Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5 (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Golgi apparatus membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in the anterior pituitary gland

Subunit

Interacts with GNAO1, GNB1 and GNG2; interaction with a heterotrimeric G protein complex is essential for receptor activity (PubMed:39103320). Interacts with GNAI2 isoform sGi2; the interaction allows the creation of an intracellular pool of DRD2 that can be released to cell surface upon agonist stimulation (PubMed:17550964). Forms homo- and heterooligomers with DRD4 (PubMed:21184734). The interaction with DRD4 may modulate agonist-induced downstream signaling (PubMed:21184734). Interacts with CADPS and CADPS2 (PubMed:15857609). Interacts with GPRASP1, PPP1R9B and CLIC6 (By similarity). Interacts with ARRB2 (By similarity). Interacts with HTR2A (PubMed:21645528). Interacts with DRD1 (By similarity). Interacts with KCNA2 (By similarity)

Gene: DRD2  |  Organism: Homo sapiens  |  Synonyms: Dopamine D2 receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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