Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1), Homo sapiens (Human), Baculovirus, N-terminal 10xHis-tagged, 75.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1)

Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1) — Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynei… Purity >85%.

SKU: BCRECP-000551 Category:
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Product Specifications

Product SkuBCRECP-000551
Product DescriptionRecombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged. It contains 1-645aa. [Accession | O14576].
Uniprot No.O14576
Gene NamesDYNC1I1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-645aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight75.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1.

Biological Context

Subcellular Location: Cytoplasm; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle pole

Product Specifications

Recombinant Human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1) is a recombinant protein from Homo sapiens (Human), expressed in Baculovirus, covering amino acids 1-645aa, with N-terminal 10xHis-tagged tag, molecular weight 75.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores

Subcellular Location

Cytoplasm; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle pole

Subunit

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1H1. Interacts with DYNLT1 and DYNLT3. Interacts with DCTN1 (By similarity). Interacts with MCRS1; the interaction is required for the proper distribution of centriolar satellites (PubMed:27263857)

Gene: DYNC1I1  |  Organism: Homo sapiens  |  Synonyms: Cytoplasmic dynein intermediate chain 1; Dynein intermediate chain 1, cytosolic
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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