Recombinant Human Elongation factor 1-alpha 1 (EEF1A1), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 54.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)

Recombinant Human Elongation factor 1-alpha 1 (EEF1A1) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-000561 Category:
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Product Specifications

Product SkuBCRECP-000561
Product DescriptionRecombinant Human Elongation factor 1-alpha 1 (EEF1A1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-462aa. [Accession | P68104].
Uniprot No.P68104
Gene NamesEEF1A1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-462aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight54.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

(Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Nucleus, nucleolus; Cell membrane

Product Specifications

Recombinant Human Elongation factor 1-alpha 1 (EEF1A1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-462aa, with N-terminal 6xHis-tagged tag, molecular weight 54.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection

Subcellular Location

Cytoplasm; Nucleus; Nucleus, nucleolus; Cell membrane

Subunit

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA (PubMed:12426392, PubMed:12426393). Interacts with PARP1 (PubMed:17177976). Interacts with KARS1 (PubMed:18029264). May interact with ERGIC2 (PubMed:17980171). Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation (PubMed:19158340). Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (PubMed:8650580). Interacts with PPP1R16B (PubMed:26497934). Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity (PubMed:18263879). Interacts with guanyl-nucleotide exchange factor EEF1B2 (By similarity). Interacts (via middle-region) with HTATIP2 (via N-terminus); the interaction is direct and competes with EEF1A1 binding to guanyl-nucleotide exchange factor EEF1B2, thereby inhibiting GDP for GTP exchange and reactivation of EEF1A1 (By similarity). Interacts with tRNA (By similarity). Interacts with CEP112 (PubMed:39349455)

Gene: EEF1A1  |  Organism: Homo sapiens  |  Synonyms: Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Leukocyte receptor cluster member 7
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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