Recombinant Human Neutrophil elastase (ELANE), Biotinylated, Homo sapiens (Human), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 73.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Neutrophil elastase (ELANE), Biotinylated

Recombinant Human Neutrophil elastase (ELANE), Biotinylated — (Microbial infection) Inhibited reversibly by S. Purity >85%.

SKU: BCRECP-000581 Categories: ,
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Product Specifications

Product SkuBCRECP-000581
Product DescriptionRecombinant Human Neutrophil elastase (ELANE) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 30-267aa. [Accession | P08246].
Uniprot No.P08246
Gene NamesELANE
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region30-267aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight73.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

(Microbial infection) Inhibited reversibly by S.aureus serine protease inhibitors Eap, EapH1 and EapH2, impairing microbicidal activity in neutrophils.

Biological Context

Subcellular Location: Cytoplasmic vesicle, phagosome; Lysosome; Cytolytic granule; Nucleus
Tissue Specificity: Bone marrow cells. Neutrophil
Disease Association: Cyclic haematopoiesis (CH) : Autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. [The disease is caused by variants affecting the gene represented in this entry] | Neutropenia, severe congenital 1, autosomal dominant (SCN1) : A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Neutrophil elastase (ELANE), Biotinylated is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 30-267aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 73.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

(Microbial infection) Inhibited reversibly by S.aureus serine protease inhibitors Eap, EapH1 and EapH2, impairing microbicidal activity in neutrophils

Subcellular Location

Cytoplasmic vesicle, phagosome; Lysosome; Cytolytic granule; Nucleus

Disease Association

Cyclic haematopoiesis (CH) : Autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. [The disease is caused by variants affecting the gene represented in this entry] | Neutropenia, severe congenital 1, autosomal dominant (SCN1) : A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Bone marrow cells. Neutrophil (PubMed:10947984)

Subunit

Interacts with NOTCH2NL (PubMed:14673143). Interacts with agaphelin, an antihemostatic protein from Anopheles gambiae (PubMed:25211214)

Gene: ELANE  |  Organism: Homo sapiens  |  Synonyms: Bone marrow serine protease; Elastase-2; Human leukocyte elastase; Medullasin; PMN elastase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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