Recombinant Human Granzyme K (GZMK), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 32.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Granzyme K (GZMK)

Recombinant Human Granzyme K (GZMK) — Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signal… Purity >90%.

SKU: BCRECP-000800 Category:
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Product Specifications

Product SkuBCRECP-000800
Product DescriptionRecombinant Human Granzyme K (GZMK) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 27-264aa. [Accession | P49863].
Uniprot No.P49863
Gene NamesGZMK
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region27-264aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight32.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway. First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization.

Biological Context

Subcellular Location: Secreted; Cell surface; Cytoplasmic granule
Tissue Specificity: Specifically expressed by CD8(+) T-cells

Product Specifications

Recombinant Human Granzyme K (GZMK) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 27-264aa, with C-terminal 6xHis-tagged tag, molecular weight 32.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:3262682, PubMed:39814882, PubMed:39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed:39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed:39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed:39814882, PubMed:39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed:24711407, PubMed:33271118, PubMed:35704599, PubMed:39614076, PubMed:39814882, PubMed:39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed:21760880, PubMed:26936634, PubMed:35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed:17308307)

Subcellular Location

Secreted; Cell surface; Cytoplasmic granule

Tissue Specificity

Specifically expressed by CD8(+) T-cells

Gene: GZMK  |  Organism: Homo sapiens  |  Synonyms: Fragmentin-3; Granzyme-3; NK-tryptase-2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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