Recombinant Human Hypoxanthine-guanine phosphoribosyltransferase (HPRT1), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 28.4kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Hypoxanthine-guanine phosphoribosyltransferase (HPRT1)

Recombinant Human Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) — Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Purity >90%.

SKU: BCRECP-000856 Categories: ,
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Product Specifications

Product SkuBCRECP-000856
Product DescriptionRecombinant Human Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-218aa. [Accession | P00492].
Uniprot No.P00492
Gene NamesHPRT1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-218aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight28.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

Biological Context

Subcellular Location: Cytoplasm
Disease Association: Lesch-Nyhan syndrome (LNS) : Characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, intellectual disability, and compulsive self-mutilation. [The disease is caused by variants affecting the gene represented in this entry] | Hyperuricemia, HPRT-related (HRH) : An X-linked metabolic disorder characterized by uric acid excess in the blood, renal stones, uric acid nephropathy, and renal obstruction. After puberty, the hyperuricemia may cause gout. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1

Product Specifications

Recombinant Human Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-218aa, with N-terminal 6xHis-tagged tag, molecular weight 28.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway

Subcellular Location

Cytoplasm

Disease Association

Lesch-Nyhan syndrome (LNS) : Characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, intellectual disability, and compulsive self-mutilation. [The disease is caused by variants affecting the gene represented in this entry] | Hyperuricemia, HPRT-related (HRH) : An X-linked metabolic disorder characterized by uric acid excess in the blood, renal stones, uric acid nephropathy, and renal obstruction. After puberty, the hyperuricemia may cause gout. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homotetramer

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1

Gene: HPRT1  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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