Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 42.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial — Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Purity >85%.

SKU: BCRECP-001242 Category:
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Product Specifications

Product SkuBCRECP-001242
Product DescriptionRecombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A). [Accession | P14543].
Uniprot No.P14543
Gene NamesNID1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A)
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight42.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane

Product Specifications

Recombinant Human Nidogen-1 (NID1) (R1085A,Y1152A,W1168A,R1194A,Y1196A), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 927-1247aa(R1085A,Y1152A,W1168A,R1194A,Y1196A), with C-terminal 6xHis-tagged tag, molecular weight 42.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane

Subunit

Interacts with FBLN1 (By similarity). Interacts with LGALS3BP (By similarity). Interacts with PLXDC1 (PubMed:16574105). Interacts with SVEP1 (By similarity)

Gene: NID1  |  Organism: Homo sapiens  |  Synonyms: Entactin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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