Recombinant Human Protein mono-ADP-ribosyltransferase PARP10 (PARP10), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Twin-Strep-tagged, 59.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Protein mono-ADP-ribosyltransferase PARP10 (PARP10), partial

Recombinant Human Protein mono-ADP-ribosyltransferase PARP10 (PARP10), partial — ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins. Purity >85%.

SKU: BCRECP-001331 Categories: ,
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Product Specifications

Product SkuBCRECP-001331
Product DescriptionRecombinant Human Protein mono-ADP-ribosyltransferase PARP10 (PARP10) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Twin-Strep-tagged. It contains 650-1025aa. [Accession | Q53GL7].
Uniprot No.Q53GL7
Gene NamesPARP10
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region650-1025aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Twin-Strep-tagged
Molecular weight59.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity.

Biological Context

Subcellular Location: Nucleus, nucleolus; Cytoplasm
Tissue Specificity: Highly expressed in spleen and thymus. Intermediate levels in liver, kidney, pancreas, prostate, testis, ovary, intestine, and leukocytes. Low expression in heart, brain, placenta, lung, skeletal muscle, and colon

Product Specifications

Recombinant Human Protein mono-ADP-ribosyltransferase PARP10 (PARP10), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 650-1025aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Twin-Strep-tagged tag, molecular weight 59.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins (PubMed:18851833, PubMed:23332125, PubMed:23474714, PubMed:25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:18851833). Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity (PubMed:23332125). Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA (PubMed:24695737)

Subcellular Location

Nucleus, nucleolus; Cytoplasm

Tissue Specificity

Highly expressed in spleen and thymus (PubMed:15674325). Intermediate levels in liver, kidney, pancreas, prostate, testis, ovary, intestine, and leukocytes (PubMed:15674325). Low expression in heart, brain, placenta, lung, skeletal muscle, and colon (PubMed:15674325)

Subunit

Interacts with MYC (PubMed:15674325). Interacts with PARP14 (PubMed:23473667). Interacts (via-PIP box and ubiquitin-interacting motifs) with PCNA (PubMed:24695737)

Gene: PARP10  |  Organism: Homo sapiens  |  Synonyms: ADP-ribosyltransferase diphtheria toxin-like 10; Poly [ADP-ribose] polymerase 10
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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