Recombinant Human Programmed cell death protein 5 (PDCD5), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 41.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Programmed cell death protein 5 (PDCD5)

Recombinant Human Programmed cell death protein 5 (PDCD5) — In response to DNA damage, associates with TP53/p53 to prevent MDM2-mediated degradation of TP53 and promote TP53-mediated signaling. Purity >90%.

SKU: BCRECP-001361 Categories: ,
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Product Specifications

Product SkuBCRECP-001361
Product DescriptionRecombinant Human Programmed cell death protein 5 (PDCD5) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-125aa. [Accession | O14737].
Uniprot No.O14737
Gene NamesPDCD5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-125aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight41.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

In response to DNA damage, associates with TP53/p53 to prevent MDM2-mediated degradation of TP53 and promote TP53-mediated signaling. Also protects KAT5/TIP60 from degradation, which further promotes TP53/p53-mediated signaling.

Biological Context

Subcellular Location: Nucleus; Cytoplasm, cytosol
Tissue Specificity: Widely expressed. Highest levels in heart, testis, kidney, pituitary gland, adrenal gland and placenta

Product Specifications

Recombinant Human Programmed cell death protein 5 (PDCD5) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-125aa, with N-terminal GST-tagged tag, molecular weight 41.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

In response to DNA damage, associates with TP53/p53 to prevent MDM2-mediated degradation of TP53 and promote TP53-mediated signaling (PubMed:19616514, PubMed:22914926, PubMed:28051100). Also protects KAT5/TIP60 from degradation, which further promotes TP53/p53-mediated signaling (PubMed:19308289)

Subcellular Location

Nucleus; Cytoplasm, cytosol

Tissue Specificity

Widely expressed. Highest levels in heart, testis, kidney, pituitary gland, adrenal gland and placenta

Subunit

Interacts (via N-terminus) with PPEF1; the interaction is direct, and leads to dephosphorylation of PDCD5 at Ser-119, PDCD5 destabilization, and downstream inhibition of TP53/p53-mediated signaling (PubMed:28051100). Interacts with TP53; the interaction is direct and prevents the interaction between TP53 and MDM2, thereby stabilizing TP53 (PubMed:22914926). Interacts with KAT5/TIP60; the interaction is direct and leads to stabilization of the KAT5 protein (PubMed:19308289)

Gene: PDCD5  |  Organism: Homo sapiens  |  Synonyms: TF-1 cell apoptosis-related protein 19
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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