Recombinant Human Phosphoglycerate kinase 1 (PGK1), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 51.5kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Phosphoglycerate kinase 1 (PGK1)

Recombinant Human Phosphoglycerate kinase 1 (PGK1) — Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Purity >95%.

SKU: BCRECP-001373 Categories: , ,
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Product Specifications

Product SkuBCRECP-001373
Product DescriptionRecombinant Human Phosphoglycerate kinase 1 (PGK1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 2-417aa. [Accession | P00558].
Uniprot No.P00558
Gene NamesPGK1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-417aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight51.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein).

Biological Context

Subcellular Location: Cytoplasm, cytosol; Mitochondrion matrix
Tissue Specificity: Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men
Disease Association: Phosphoglycerate kinase 1 deficiency (PGK1D) : A condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations, and some affected individuals develop parkinsonian symptoms. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5

Product Specifications

Recombinant Human Phosphoglycerate kinase 1 (PGK1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-417aa, with C-terminal 6xHis-tagged tag, molecular weight 51.5kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed:18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed:26942675, PubMed:36849569). May play a role in sperm motility (PubMed:26677959)

Subcellular Location

Cytoplasm, cytosol; Mitochondrion matrix

Disease Association

Phosphoglycerate kinase 1 deficiency (PGK1D) : A condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations, and some affected individuals develop parkinsonian symptoms. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men

Subunit

Monomer (PubMed:18463139). Interacts with kinase MAPK1/ERK2; the interaction is direct, occurs under hypoxic conditions, and promotes its interaction with PIN1 (PubMed:26942675). Interacts with peptidyl-prolyl cis-trans isomerase PIN1; the interaction is direct, occurs under hypoxic conditions, and targets the protein to the mitochondrion by promoting interactions with the TOM complex (PubMed:26942675). Interacts with mitochondrial circRNA mcPGK1 (via its 2nd stem-loop); the interaction is direct and targets the protein to the mitochondrion by promoting interactions with the TOM complex (PubMed:36849569). Interacts with pyruvate dehydrogenase kinase PDK1; the interaction is direct, occurs under hypoxic conditions and leads to PDK1-mediated inhibition of pyruvate dehydrogenase complex activity (PubMed:26942675)

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5

Gene: PGK1  |  Organism: Homo sapiens  |  Synonyms: Cell migration-inducing gene 10 protein; Primer recognition protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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