Recombinant Human Podocalyxin (PODXL), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 48.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Podocalyxin (PODXL), partial

Recombinant Human Podocalyxin (PODXL), partial — Involved in the regulation of both adhesion and cell morphology and cancer progression. Purity >90%.

SKU: BCRECP-001412 Category:
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Product Specifications

Product SkuBCRECP-001412
Product DescriptionRecombinant Human Podocalyxin (PODXL) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 32-458aa. [Accession | O00592].
Uniprot No.O00592
Gene NamesPODXL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region32-458aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight48.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner.

Biological Context

Subcellular Location: Apical cell membrane; Cell projection, lamellipodium; Cell projection, filopodium; Cell projection, ruffle; Cell projection, microvillus; Membrane raft; Membrane (Single-pass type I membrane protein)
Tissue Specificity: Glomerular epithelium cell (podocyte)

Product Specifications

Recombinant Human Podocalyxin (PODXL), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 32-458aa, with N-terminal 6xHis-tagged tag, molecular weight 48.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells

Subcellular Location

Apical cell membrane; Cell projection, lamellipodium; Cell projection, filopodium; Cell projection, ruffle; Cell projection, microvillus; Membrane raft; Membrane (Single-pass type I membrane protein)

Tissue Specificity

Glomerular epithelium cell (podocyte)

Subunit

Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF1 (via the PDZ domains); the interaction is not detected in glomerular epithelium cells, take place early in the secretory pathway and is necessary for its apical membrane sorting. Found in a complex with EZR, PODXL and NHERF2. Associates with the actin cytoskeleton through complex formation with EZR and NHERF2. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells (By similarity). Interacts with EZR

Gene: PODXL  |  Organism: Homo sapiens  |  Synonyms: GCTM-2 antigen; Gp200; Podocalyxin-like protein 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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