Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP), Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 80.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP)

Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP) — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-001518 Category:
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Product Specifications

Product SkuBCRECP-001518
Product DescriptionRecombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-663aa. [Accession | P47736].
Uniprot No.P47736
Gene NamesRAP1GAP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-663aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight80.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Biological Context

Subcellular Location: Golgi apparatus membrane (Peripheral membrane protein)
Tissue Specificity: Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues

Product Specifications

Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-663aa, with C-terminal 6xHis-tagged tag, molecular weight 80.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state

Subcellular Location

Golgi apparatus membrane (Peripheral membrane protein)

Tissue Specificity

Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues

Subunit

Homodimer and heterodimer with RAP1B

Gene: RAP1GAP  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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