Recombinant Human Protein CBFA2T1 (RUNX1T1), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 75kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Protein CBFA2T1 (RUNX1T1)

Recombinant Human Protein CBFA2T1 (RUNX1T1) — Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Purity >85%.

SKU: BCRECP-001571 Category:
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Product Specifications

Product SkuBCRECP-001571
Product DescriptionRecombinant Human Protein CBFA2T1 (RUNX1T1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-604aa. [Accession | Q06455].
Uniprot No.Q06455
Gene NamesRUNX1T1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-604aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight75kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Can repress transactivation mediated by TCF12.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary
Disease Association: [A chromosomal aberration involving RUNX1T1 is frequently found in acute myeloid leukemia with maturation (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1 that results in the production of AML1-MTG8/ETO fusion protein]

Product Specifications

Recombinant Human Protein CBFA2T1 (RUNX1T1) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-604aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 75kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:10688654, PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Can repress transactivation mediated by TCF12 (PubMed:16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588)

Subcellular Location

Nucleus

Disease Association

[A chromosomal aberration involving RUNX1T1 is frequently found in acute myeloid leukemia with maturation (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1 that results in the production of AML1-MTG8/ETO fusion protein]

Tissue Specificity

Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary

Subunit

Homooligomer. Homotetramerization is mediated by nervy homology region 2 (NRH2). Can interact with CBFA2T2 and CBFA2T3; heterotetramerization between members of the CBFA2T family is proposed. Interacts with TCF12, SIN3A, HDAC1, HDAC2, HDAC3, NCOR1, NCOR2. Interacts with ATN1 (via its N-terminus); the interaction enhances the transcriptional repression. Interacts (via its N-terminus) with ZBTB16; the interaction increases the transcription repression activity of ZBTB16 (PubMed:10688654). AML1-MTG8/ETO fusion protein interacts with CBFB. AML1-MTG8/ETO is part of a stable transcription factor complex AETFC in leukemic cells; AETFC formation seems to be involved in recruitment of EP300. AML1-MTG8/ETO nervy homology region 2-mediated oligomerization is proposed to be homotypic, required for AML1-MTG8/ETO-mediated transformation of primary hematopoietic cells and is required for AML1-MTG8/ETO interaction with TCF12

Gene: RUNX1T1  |  Organism: Homo sapiens  |  Synonyms: Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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