Recombinant Human Antithrombin-III (SERPINC1) (R294C), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 58.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Antithrombin-III (SERPINC1) (R294C)

Recombinant Human Antithrombin-III (SERPINC1) (R294C) — Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Purity >85%.

SKU: BCRECP-001616 Category:
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Product Specifications

Product SkuBCRECP-001616
Product DescriptionRecombinant Human Antithrombin-III (SERPINC1) (R294C) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-464aa(R294C). [Accession | P01008].
Uniprot No.P01008
Gene NamesSERPINC1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-464aa(R294C)
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight58.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

Biological Context

Subcellular Location: Secreted, extracellular space
Tissue Specificity: Found in plasma
Disease Association: Antithrombin III deficiency (AT3D) : An important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. Antithrombin-III deficiency is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Antithrombin-III (SERPINC1) (R294C) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-464aa(R294C), with N-terminal 6xHis-tagged tag, molecular weight 58.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade (PubMed:15140129, PubMed:15853774). AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa (PubMed:15140129). Its inhibitory activity is greatly enhanced in the presence of heparin

Subcellular Location

Secreted, extracellular space

Disease Association

Antithrombin III deficiency (AT3D) : An important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. Antithrombin-III deficiency is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Found in plasma

Subunit

Forms protease inhibiting heterodimer with TMPRSS7

Gene: SERPINC1  |  Organism: Homo sapiens  |  Synonyms: Serpin C1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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