Recombinant Human YTH domain-containing family protein 2 (YTHDF2), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 68.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human YTH domain-containing family protein 2 (YTHDF2)

Recombinant Human YTH domain-containing family protein 2 (YTHDF2) — (Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK. Purity >85%.

SKU: BCRECP-001927 Category:
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Product Specifications

Product SkuBCRECP-001927
Product DescriptionRecombinant Human YTH domain-containing family protein 2 (YTHDF2) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 2-579aa. [Accession | Q9Y5A9].
Uniprot No.Q9Y5A9
Gene NamesYTHDF2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-579aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight68.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

(Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cytoplasm, P-body; Cytoplasm, Stress granule; Nucleus
Tissue Specificity: Highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation

Product Specifications

Recombinant Human YTH domain-containing family protein 2 (YTHDF2) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-579aa, with N-terminal 10xHis-tagged tag, molecular weight 68.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells) (PubMed:29659627). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs (PubMed:29659627)

Subcellular Location

Cytoplasm, cytosol; Cytoplasm, P-body; Cytoplasm, Stress granule; Nucleus

Tissue Specificity

Highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation

Subunit

Interacts with CNOT1; interaction is direct and promotes recruitment of the CCR4-NOT complex (PubMed:27558897, PubMed:32492408). Interacts with YTHDF3 (PubMed:28106072). Interacts with RIDA/HRSP12; interaction leads to recruitment of the ribonuclease P/MRP complex (PubMed:30930054)

Gene: YTHDF2  |  Organism: Homo sapiens  |  Synonyms: CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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