Recombinant Rat Mannose-binding protein C (Mbl2), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-tagged, 30.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Mannose-binding protein C (Mbl2)

Recombinant Rat Mannose-binding protein C (Mbl2) — Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of … Purity >90%.

SKU: BCRECP-003363 Category:
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Product Specifications

Product SkuBCRECP-003363
Product DescriptionRecombinant Rat Mannose-binding protein C (Mbl2) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 19-244aa. [Accession | P08661].
Uniprot No.P08661
Gene NamesMbl2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region19-244aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight30.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway.

Biological Context

Subcellular Location: Secreted; Cell surface

Product Specifications

Recombinant Rat Mannose-binding protein C (Mbl2) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 19-244aa, with N-terminal 6xHis-tagged tag, molecular weight 30.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:11850428, PubMed:3009480, PubMed:8557671). Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed:11850428, PubMed:8557671)

Subcellular Location

Secreted; Cell surface

Subunit

Oligomeric complex of 3 or more homotrimers (PubMed:11850428, PubMed:22167201, PubMed:9922165). Interacts with MASP1 and MASP2 (By similarity). Interacts with MEP1A and MEP1B and may inhibit their catalytic activity (By similarity). Interacts with CR1 (via Sushi 24 and Sushi 25 domains) (By similarity)

Gene: Mbl2  |  Organism: Rattus norvegicus  |  Synonyms: Mannan-binding protein; Ra-reactive factor polysaccharide-binding component p28A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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