Recombinant Mouse Macrophage migration inhibitory factor (Mif) — Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. Purity >90%.
| Product Sku | BCRECP-003389 |
| Product Description | Recombinant Mouse Macrophage migration inhibitory factor (Mif) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 2-115aa. [Accession | P34884]. |
| Uniprot No. | P34884 |
| Gene Names | Mif |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 2-115aa |
| Species | Mus musculus (Mouse) |
| Tag Info | N-terminal 10xHis-tagged |
| Molecular weight | 18.4kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Immunology |
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense.
Recombinant Mouse Macrophage migration inhibitory factor (Mif) — Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. Purity >90%. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity). Counteracts the anti-inflammatory activity of glucocorticoids (By similarity). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:10933783, PubMed:16780921, PubMed:19188446). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:10933783, PubMed:16780921, PubMed:19188446)
Secreted; Cytoplasm
Homotrimer (PubMed:16780921). Interacts with CD74 and CXCR2 extracellular domain and COPS5 (PubMed:19188446). Interacts with the USO1 and BNIPL (By similarity)
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
