Recombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA), Escherichia coli (strain K12), E.coli, N-terminal 6xHis-SUMO-tagged, 73.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA)

Recombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA) — Part of the ABC transporter complex MppA-OppBCDF involved in the uptake of the cell wall murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Purity >90%.

SKU: BCRECP-003408 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003408
Product DescriptionRecombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 23-537aa. [Accession | P77348].
Uniprot No.P77348
Gene NamesmppA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region23-537aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight73.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Part of the ABC transporter complex MppA-OppBCDF involved in the uptake of the cell wall murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Is involved in the recycling of cell wall peptides.

Product Specifications

Recombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA) — Part of the ABC transporter complex MppA-OppBCDF involved in the uptake of the cell wall murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Part of the ABC transporter complex MppA-OppBCDF involved in the uptake of the cell wall murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate (PubMed:9495761). Is involved in the recycling of cell wall peptides (PubMed:9495761). Binds the cell wall peptide L-Ala-D-Gly-gamma-meso-diaminopimelic acid (PubMed:21705338, PubMed:9495761). Can also transport ordinary alpha-linked tripeptides such as Pro-Phe-Lys, but with much lower efficiency than OppA (PubMed:9495761). Cannot bind typical tripeptides such as Lys-Glu-Lys, Lys-Lys-Lys or Ala-Ala-Ala (PubMed:21705338)

Subcellular Location

Periplasm

Subunit

The complex is composed of two ATP-binding proteins (OppD and OppF), two transmembrane proteins (OppB and OppC) and a solute-binding protein (MppA)

Gene: mppA  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Murein peptide permease A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top