Recombinant Lucilia sericata Lucifensin, Lucilia sericata (Green bottle fly) (Phaenicia sericata), E.coli, N-terminal 6xHis-KSI-tagged, 19.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Lucilia sericata Lucifensin

Recombinant Lucilia sericata Lucifensin — Shows strong antibacterial activity against numerous Gram-positive bacteria. Purity >90%.

SKU: BCRECP-003565 Category:
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Product Specifications

Product SkuBCRECP-003565
Product DescriptionRecombinant Lucilia sericata Lucifensin Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 53-92aa. [Accession | P86471].
Uniprot No.P86471
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region53-92aa
SpeciesLucilia sericata (Green bottle fly) (Phaenicia sericata)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight19.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Shows strong antibacterial activity against numerous Gram-positive bacteria. It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio) thus inhibiting cell wall synthesis. Shows antibacterial activity against the Gram-positive bacteria M.luteus, E.fecalis (MIC=32 mg/L), S.aureus (MIC=16 mg/L), S.carnosus (MIC=2 mg/L), S.pneumoniae (MIC=2 mg/L) and S.pyogenes (MIC=2 mg/L) and against a number of methicillin-resistant S.aureus and glycopeptide-intermediate S.aureus isolates.

Biological Context

Subcellular Location: Secreted; Host cell membrane
Tissue Specificity: Larval fat body, hemolymph and salivary glands (at protein level). Expressed in the salivary glands of all larval stages

Product Specifications

Recombinant Lucilia sericata Lucifensin is a recombinant protein from Lucilia sericata (Green bottle fly) (Phaenicia sericata), expressed in E.coli, covering amino acids 53-92aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 19.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Shows strong antibacterial activity against numerous Gram-positive bacteria. It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio) thus inhibiting cell wall synthesis (PubMed:20508130). Shows antibacterial activity against the Gram-positive bacteria M.luteus, E.fecalis (MIC=32 mg/L), S.aureus (MIC=16 mg/L), S.carnosus (MIC=2 mg/L), S.pneumoniae (MIC=2 mg/L) and S.pyogenes (MIC=2 mg/L) and against a number of methicillin-resistant S.aureus and glycopeptide-intermediate S.aureus isolates (PubMed:19921400, PubMed:20542901, PubMed:21560219). Does not show antibacterial activity against Gram-negative bacteria or antifungal activity against C.utilis (PubMed:20542901). Shows slight antifungal activity against C.albicans (PubMed:21560219)

Subcellular Location

Secreted; Host cell membrane

Tissue Specificity

Larval fat body, hemolymph and salivary glands (at protein level) (PubMed:19921400). Expressed in the salivary glands of all larval stages (PubMed:23624615)

Organism: Lucilia sericata  |  Synonyms: Sericasin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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