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Recombinant Allium sativum Alliin lyase 1

Recombinant Allium sativum Alliin lyase 1 — recombinant protein from Allium sativum (Garlic), expressed in E.coli. Purity >90%.

SKU: BCRECP-003571 Categories: ,

Product Specifications

Product SkuBCRECP-003571
Product DescriptionRecombinant Allium sativum Alliin lyase 1 Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 39-486aa. [Accession | Q01594].
Uniprot No.Q01594
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region39-486aa
SpeciesAllium sativum (Garlic)
Tag InfoC-terminal 6xHis-tagged
Molecular weight58.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering.

Biological Context

Subcellular Location: Vacuole
Tissue Specificity: Expressed in bulb (at protein level). Expressed in shoots

Product Specifications

Recombinant Allium sativum Alliin lyase 1 is a recombinant protein from Allium sativum (Garlic), expressed in E.coli, covering amino acids 39-486aa, with C-terminal 6xHis-tagged tag, molecular weight 58.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering

Subcellular Location

Vacuole

Tissue Specificity

Expressed in bulb (at protein level) (PubMed:12136147, PubMed:12235163, PubMed:17174334). Expressed in shoots

Subunit

Homodimer

Organism: Allium sativum  |  Synonyms: Cysteine sulphoxide lyase 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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